Cell–Matrix Interactions Improve β-Cell Survival and Insulin Secretion in Three-Dimensional Culture

three-dimensional (3d) cell culture conditions, present and future improvements

conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...

Lipases in the pancreatic β-cell: implications for insulin secretion

Lipids have been implicated inβ-cell stimulus–secretion coupling. In such a role, lipases inβ-cellswould be required to generate lipid coupling factors. We have shown previously that glucose stimulates lipolysis in rodent islets. In addition, lipolysis and diacylglycerol lipase activity in islets are abolished by orlistat, an irreversible lipase inhibitor with a broad specificity for substrates...

Three-dimensional microwell arrays for cell culture.

We propose the concept of three-dimensional (3D) microwell arrays for cell culture applications and highlight the importance of oxygen diffusion through pores in all three dimensions to enhance cell viability.

Three-dimensional Structural Interactions of Insulin and Its Receptor*□S

The insulin receptor (IR) belongs to the superfamily of transmembrane receptor tyrosine kinases (TKs) (reviewed in Ref. 1). In contrast to other family members that are monomeric in their structure, IR and its homologue, insulin-like growth factor I receptor (IGF-1R), are intrinsic disulfide-linked dimers of heterodimeric disulfide-linked proteins of the form ( )2. The 135-kDa subunit of IR is ...

Three-dimensional structural interactions of insulin and its receptor.